Gentamicin bioautography assay vs. the microbiological disk test.
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چکیده
منابع مشابه
Four-hour microbiological assay of gentamicin in serum.
Since the microbiological assay of the antibiotic content of serum generally requires 18 to 24 hr of incubation, results of such procedures may not become available in time to make appropriate adjustments in subsequent dosages of antibiotic. A 4-hr bioassay for determining concentrations of gentamicin in serum has been developed in which Staphylococcus aureus ATCC 6538P is used as the test orga...
متن کاملComparison of three experimental designs employed in gentamicin microbiological assay through agar diffusion
Gentamicin is a broad-spectrum antibiotic complex produced by actinomycetes belonging to Micromonospora genus and classified among aminoglycoside antibiotics, used in the treatment of serious infections derived from Gram-negative microorganisms. Alterations of their antimicrobial activity not shown in chemical assays can be evaluated through microbiological assays. The aim of this work was to c...
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Although vitamin B12 can be assayed biologically with mice, chicks or rats, the microbiological method of assay is preferred since it is more rapid and economical. One serious shortcoming of many microbiological B12 assay procedures is the lack of specificity when crude materials are assayed. Crude materials can contain inhibitory or stimulatory factors which cause serious errors in assay resul...
متن کاملA spectrophotometric assay for gentamicin.
A rapid and accurate spectrophotometric assay has been developed for the determination of blood werum levels of gentamicin and related antibiotics. The assay uses a purified enzyme from Escherichia coli JR88/C600 that acetylates gentamicin with the production of coenzyme A, linked to a chemical reaction with a sulfhydryl reagent to produce stoichiometric amounts of a sensitive chromophore, moni...
متن کاملEvaluation of the Bactec serum gentamicin assay.
A radiometric assay for gentamicin was compared with an established radioimmunoassay protocol. The coefficients of variation for within-run, day-to-day, and overall runs were consistently higher for the radiometric method as compared with the radioimmunoassay. The coefficient of correlation for 84 patient sera tested by the two methods for gentamicin levels was 0.82. Though less precise, the ra...
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ژورنال
عنوان ژورنال: The Journal of Antibiotics
سال: 1980
ISSN: 0021-8820,1881-1469
DOI: 10.7164/antibiotics.33.600